Translating Mass Spectrometry - Based Proteomics of Malignant Lymphoma into Clinical Application Megan
نویسنده
چکیده
The recent development of multidimensional liquid chromatographic methods combined with tandem mass spectrometry (LC-LC-MS/MS) has permitted sensitive detection of thousands of low abundance proteins, membrane proteins and proteins with extreme isoelectric points (pI) from highly complex protein mixtures. The ability to perform global relative quantitative proteomics has been significantly enhanced by the advent of numerous isotope labeling approaches that are efficient in simplifying the proteome, and in combination with LC-MS/MS permit detection and quantification of proteins and peptides from very complex samples.
منابع مشابه
Mass spectrometry-based proteomic studies of human anaplastic large cell lymphoma.
Malignant lymphomas are a diverse group of malignant neoplasms that arise as a result of a complex interplay of multiple factors including genetic aberrations, immunosuppression, and exposure to noxious agents such as ionizing radiation and chemical agents. Anaplastic large cell lymphoma (ALCL) is an aggressive T-lineage lymphoma harboring chromosomal translocations involving the anaplastic lym...
متن کاملProteomic identification of oncogenic chromosomal translocation partners encoding chimeric anaplastic lymphoma kinase fusion proteins.
The anaplastic lymphoma kinase (ALK) on 2p23 is a tyrosine kinase that forms chimeric fusions with numerous translocation partners. We describe a mass spectrometry-based approach for the identification of ALK fusion partners. This approach accurately identified the nucleophosmin (NPM)-ALK fusion protein in an anaplastic large cell lymphoma (ALCL)-derived cell line carrying the t(2;5)(p23;q35), ...
متن کاملPrimary Malignant Lymphoma of Cervix: A Case Report
Background:Primary malignant lymphoma of the uterine cervix is extremely rare and can be misdiagnosed due to its different clinical presentations. The aim of this report was to introduce a case of primary malignant lymphoma of the uterine cervix which was misdiagnosed as an ovarian cyst on ultrasonography. Case report: A 31-year-old female referred with a...
متن کاملProteomics insights into DNA damage response and translating this knowledge to clinical strategies
Genomic instability is a critical driver in the process of cancer formation. At the same time, inducing DNA damage by irradiation or genotoxic compounds constitutes a key therapeutic strategy to kill fast-dividing cancer cells. Sensing of DNA lesions initiates a complex set of signalling pathways, collectively known as the DNA damage response (DDR). Deciphering DDR signalling pathways with high...
متن کاملMalignant Lymphoma of Lacrimal Apparatus in the 7 Year Old Girl
Abstract Background Involvement of lacrimal apparatus by Non Hodgkin lymphoma as a first manifestation is extremely rare and only three cases have been reported in children. The purpose of this report is to determine the clinical characteristic of patient with primary involvement of lacrimal apparatus with lymphoma. Case presentation A 7-year-old girl with history of tearing, gradually prog...
متن کامل